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"content": "<p>Turns out the postsynaptic densities (PSD) that we use in electron microscopy to identify synapses aren’t made of large protein aggregates but rather of specialised cellular organelles:</p><p>“we combined mouse genetics and cryo-electron tomography to determine the 3D molecular architecture of fresh isolated and anatomically intact synapses in the adult forebrain. The native glutamatergic synapse did not consistently show a high density of proteins at the postsynaptic membrane thought to be characteristic of the PSD. Instead, a ‘synaptoplasm’ consisting of cytoskeletal elements, macromolecular complexes and membrane-bound organelles extended throughout the pre- and post-synaptic compartments.”</p><p>Excellent work by Julia Peukes et al. 2024 <a href=\"https://elifesciences.org/reviewed-preprints/100335\" target=\"_blank\" rel=\"nofollow noopener noreferrer\" translate=\"no\"><span class=\"invisible\">https://</span><span class=\"ellipsis\">elifesciences.org/reviewed-pre</span><span class=\"invisible\">prints/100335</span></a></p><p><a href=\"https://mathstodon.xyz/tags/neuroscience\" class=\"mention hashtag\" rel=\"tag\">#<span>neuroscience</span></a> <a href=\"https://mathstodon.xyz/tags/synapses\" class=\"mention hashtag\" rel=\"tag\">#<span>synapses</span></a> <a href=\"https://mathstodon.xyz/tags/CryoEM\" class=\"mention hashtag\" rel=\"tag\">#<span>CryoEM</span></a> <a href=\"https://mathstodon.xyz/tags/CryoCLEM\" class=\"mention hashtag\" rel=\"tag\">#<span>CryoCLEM</span></a></p>",
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"en": "<p>Turns out the postsynaptic densities (PSD) that we use in electron microscopy to identify synapses aren’t made of large protein aggregates but rather of specialised cellular organelles:</p><p>“we combined mouse genetics and cryo-electron tomography to determine the 3D molecular architecture of fresh isolated and anatomically intact synapses in the adult forebrain. The native glutamatergic synapse did not consistently show a high density of proteins at the postsynaptic membrane thought to be characteristic of the PSD. Instead, a ‘synaptoplasm’ consisting of cytoskeletal elements, macromolecular complexes and membrane-bound organelles extended throughout the pre- and post-synaptic compartments.”</p><p>Excellent work by Julia Peukes et al. 2024 <a href=\"https://elifesciences.org/reviewed-preprints/100335\" target=\"_blank\" rel=\"nofollow noopener noreferrer\" translate=\"no\"><span class=\"invisible\">https://</span><span class=\"ellipsis\">elifesciences.org/reviewed-pre</span><span class=\"invisible\">prints/100335</span></a></p><p><a href=\"https://mathstodon.xyz/tags/neuroscience\" class=\"mention hashtag\" rel=\"tag\">#<span>neuroscience</span></a> <a href=\"https://mathstodon.xyz/tags/synapses\" class=\"mention hashtag\" rel=\"tag\">#<span>synapses</span></a> <a href=\"https://mathstodon.xyz/tags/CryoEM\" class=\"mention hashtag\" rel=\"tag\">#<span>CryoEM</span></a> <a href=\"https://mathstodon.xyz/tags/CryoCLEM\" class=\"mention hashtag\" rel=\"tag\">#<span>CryoCLEM</span></a></p>"
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